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Elabscience Biotechnology
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Elabscience Biotechnology
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Applygen Technologies
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Beijing Solarbio Science
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Neogen
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Image Search Results
Journal: Allergologia et immunopathologia
Article Title: Alpinia officinarum Hance extract relieved sepsis-induced myocardial ferroptosis and inflammation by inhibiting lncRNA MIAT/TRAF6/NF-κB axis.
doi: 10.15586/aei.v52i5.1035
Figure Lengend Snippet: Figure 2 Alpinia officinarum Hance extract inhibited sepsis-induced myocardial ferroptosis. Groups were divided into the Sham, LPS, LPS+25 mg/kg AOE, LPS+50 mg/kg AOE, and LPS+100 mg/kg AOE groups. (A) The Fe2+ level was evaluated through the corresponding commercial kit. (B) The levels of MDA and SOD were examined through ELISA. (C) The protein expressions of ACSL4 and GPX4 were tested through western blot.
Article Snippet: Detection of Fe2+ The
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot
Journal: Allergologia et immunopathologia
Article Title: Alpinia officinarum Hance extract relieved sepsis-induced myocardial ferroptosis and inflammation by inhibiting lncRNA MIAT/TRAF6/NF-κB axis.
doi: 10.15586/aei.v52i5.1035
Figure Lengend Snippet: Figure 5 Overexpression of MIAT reduced the cardioprotective effect of Alpinia officinarum Hance extract. Groups were separated into the Sham, LPS, LPS+100 mg/kg AOE, and LPS+100 mg/kg+Ad-MIAT groups. (A) The protein expressions of TRAF6, p-P65, and P65 were verified through western blot. (B) The level of CK-MB in serum was examined through ELISA. (C) The level of cTnl in serum was measured through ELISA. (D) The Fe2+ level was tested through the corresponding commercial kit. (E) The levels of MDA and SOD were measured through ELISA.
Article Snippet: Detection of Fe2+ The
Techniques: Over Expression, Western Blot, Enzyme-linked Immunosorbent Assay
Journal: Viruses
Article Title: Infectious Spleen and Kidney Necrosis Virus Triggers Ferroptosis in CPB Cells to Enhance Virus Replication.
doi: 10.3390/v17050713
Figure Lengend Snippet: Figure 3. ISKNV infection induces ferroptosis in CPB cells. (A) Transmission electron microscopy of CPB cells treated with DMSO (72 h), erastin (10 µmol/L, 72 h), and ISKNV (100 MOI, 24 h, 48 h, 72 h). Scale bars = 1 µm. (B) Analysis of Fe2+ levels in CPB cells after treatment with ISKNV (100 MOI) using the fluorescent probe FerroOrange by laser scanning confocal microscopy. Scale bars = 20 µm. (C) Quantitative analysis of the mean fluorescence intensity of (B) using Image J. (D) Analysis of intracellular ROS levels using DCFH-DA staining, and laser scanning confocal microscopy of CPB cells treated with ISKNV (100 MOI) for 24–72 h. Scale bars = 10 µm. (E) Quantitative analysis of the mean fluorescence intensity of (D) using Image J. (F–H) Detection of Fe2+, ROS, and MDA levels in cell lysates treated with ISKNV (100 MOI) for 24–72 h by microplate reader. * p < 0.05, ** p < 0.01, and *** p < 0.001, with p > 0.05 considered not significant (ns).
Article Snippet: The Fe2+ content of the cells was detected by laser scanning confocal microscopy and a microplate reader using the
Techniques: Infection, Transmission Assay, Electron Microscopy, Confocal Microscopy, Fluorescence, Staining